Knowledge-enriched characterization of mobile populations. Morphological facts from photographs can increase on the richness of flow cytometry details. Such as, the figure shows an in any other case regular apoptosis assay working with Annexin V and PI, introducing mobile imaging to characterize cells in Each and every populace to reveal morphologically distinctive capabilities. These insights couldn't have been attained from multiplex staining by itself.
As samples are obtained within the Attune CytPix Flow Cytometer, the superior-speed brightfield digital camera captures and shops visuals of detected functions in a price as much as six,000 photos for each next, dependant upon the flow level and image measurement. For higher flexibility, Attune Cytometric Software enables you to adjust the image capture frequency as necessary and select a certain gate for picture seize, if necessary.
(B) RAW 264.7 macrophages are sometimes annotated inaccurately with respect to particle depend due to their complexity and noncircular borders. The number of singlets readily available for Investigation improves when the person trains and employs a more correct design.
Learn new insights into your experimental information by visualizing populations utilizing blended fluorescence and morphology information. Our application interface wizards help downsampling, concatenation (merged information) and dimensionality instruments which are built in to the program for an intuitive, streamlined details Evaluation practical experience.
"A picture is just not very truly worth one thousand dots, but it seriously helps you to see illustrations or photos. It is excellent to see photographs that correlate with staining. By way of example, AnnexinV-positive cells which have been morphologically diverse than healthier cells, or CD14+ monocytes which are bigger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Additionally, it truly is illuminating to find out the amount of doublets slip via regular singlet gates, and terrific to confirm the position of unpredicted double-beneficial occasions in my Evaluation."
Optimizing gating system. Even strong handbook singlet gating is mistake-vulnerable and remains a subjective selection position in Just about all flow cytometry assays. Imaging can be utilized to verify and regulate gates to include only solitary cells of curiosity.
Our scientists will design and style panels for you personally as over and over you'll need—free of charge, no product or service purchase required.
The Attune NxT cytometer will be the reliable workhorse instrument your lab can depend upon for consistent and trusted efficiency. The Attune CytPix model features a significant-pace brightfield digicam and AI-driven graphic analysis software program, with current upgrades together with device learning equipment and an intuitive person interface, enabling enhanced info precision, precision, and faster insights into sample biology.
Consumers can view celebration photographs in true-time to substantiate sample quality, optimize protocols, and report the morphology of each mobile populace recognized for analysis. Imaging seize also Rewards from acoustic focusing, which helps to situation the cells making sure that a pointy, centered image is received.
Sound waves help exact cell alignment, leading to Extraordinary knowledge quality and ~10X faster sample flow prices.
Application Observe: Automated graphic Assessment reduces user-to-consumer variability in flow cytometry gating techniques
Built-in dimensionality reduction tools greater resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to substantiate expected populace resolution in lysed whole human blood. Person can check out gatherings from Every single cluster for Visible affirmation of cell phenotypes.
No wash/no lyse leukocyte Investigation with imaging. Cells ended check here up acquired from 24-hour-aged blood by dilution in one mM EDTA ( Specialist UX/UI allows customers to teach AI styles for expanded purposes. (A) The “Teach Product” function gives annotation instruments and serious-time model training precision.
Gating on these extended parameters helps you to swiftly and accurately establish populations of interest and ensure gating approach with little if any guide assessment. The Attune computer software features entry to point out-of-the-art equipment Understanding Evaluation applications in just an expertly created person interface, which makes it extremely user friendly.
The Attune Xenith cytometer is a higher-parameter spectral analyzer, offering both equally spectral unmixing and traditional flow abilities for extensive and adaptable cell Evaluation.
The Attune CytPix Flow Cytometer enables users to visualize samples for the duration of acquisition, validate the precision of gating strategies, and assist success with reputable documentation for publication applications.
What gave the impression to be a homogeneous inhabitants is actually extra assorted—an insight that needs to be regarded as when interpreting final results.